Leica DMi8 basic fluorescence widefield microscope

Leica DMi8 basic equipment details

Standard widefield microscope for fluorescence-based imaging

This system is based on an inverted Leica DMi8 microscope that is equipped with a Sola II LED light source and four standard filter cubes (see below). The camera for monochrome image acquisition is a Leica DFC3000 G with a full frame size of 1296 pixels x 966 pixels. The XY-stage is manually operated, whereas the Z-drive is motorised. The system is located is in room C2.38.

Objectives

The DMi8 is equipped with the following lenses:

Lens

magnif.    

corr      

NA

N PLAN

10x

A

0.25

N PLAN L

20x

A

0.4

HCX PL FLUOTAR L Ph2       

40x

A

0.6

HCX PL APO

63x

O

1.3

 

A = air, O = immersion oil

 

Filter sets

The following filter sets for epifluorescence illumination are available on the DMi8:

Fluorophore     

exc                

 

em

DAPI

405/10

 

460/40

FITC

470/40

 

525/50

TexR

560/40   LP610

Cy5

620/60

 

700/75