Equipment details and manuals Confocal laser scanning microscope for multi-dimensional image acquisition and live cell imaging This system is based on an inverted DMI 6000 CS microscope base, and the scanhead (SP5C) is equipped with three photo multiplier tubes (Hamamatsu R 9624). The system is also equipped with an environment control chamber to maintain environmental conditions for live cell imaging (temperature, 5% carbon dioxide concentration and air humidity). 5 lasers, including a 405 nm blue diode laser, provide 9 different laser lines and thus high flexibility for fluorophore excitation. The system includes LED-based epifluorescence illumination with DAPI, FITC and Rhodamin filter cubes (see below). Lasers The SP5 provides the following lasers with the specified laser lines for fluorophore excitation: Laser lines [nm] Arg 458 476 488 496 514 HeNe 1 543 HeNe 2 594 HeNe3 633 Blue diode 405 Objectives The SP5 is equipped with the following lenses: Lens magnification immersion NA HCX PL APO, 0.17CS 63x W 1.2 HC PL APO, CS 10x A 0.4 HC PLAN APO, PH2 0.17/C 0.59 20x A 0.7 HCX PL APO PH3 CS 40x O 1.25 HCX PL APO, lbd. BL 63x O 1.4 PI APO 100x O 1.4 W = water, A = air, O = oil Filter sets The following filter sets for epifluorescence illumination are available on the SP5: A UV BP340-380 400 LP 425; I3 blue BP 450-490 510 LP 515; N2.1 green BP 515-560 580 LP 590 For more technical details of the SP5 system, please refer to the technical documentation below. Optional: Manuals for the operation of the Leica SP5 This website provides 3 different manuals that describe how to operate the SP5: CALM SP5 manual - This is an illustrated manual that explains how to use the system in step-by-step protocols. Click the button to acces the manual: Document CALM Leica TCS SP5 user manual (2.02 MB / PDF) LeicaDMI6000 user manual - This manual by Leica Microsystems gives a detailed description of the DMI6000 inverted microscope base Document Leica DMI6000B manual (1.1 MB / PDF) Start-up and run-down procedures Quick guide for step-by-step protocols for the start-up and closing-down of the SP5 are given at the following link: Document Leica TCS SP5 quick start guide (98 KB / PDF) This article was published on 2024-08-22
